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Article

Changes in apparent free energy of helix-helix dimerization in a biological membrane due to point mutations.

Journal of molecular biology - 10 Aug 2007

Duong Mylinh T, Jaszewski Todd M, Fleming Karen G, MacKenzie Kevin R

Abstract excerpt

We present an implementation of the TOXCAT membrane protein self-association assay that measures the change in apparent free energy of transmembrane helix dimerization caused by point mutations. Quantifying the reporter gene expression from cells carrying wild-type and mutant constructs shows that single point mutations that disrupt dimerization of the transmembrane domain of glycophorin A reproducibly lower the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 17570394
DOI: 10.1016/j.jmb.2007.05.026
PMCID: PMC2693063

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