Article
[wt-p53 gene repair for p53 mutations of salivary pleomorphic adenoma cells].
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology - 1 Mar 2007
Wang Xu, Wang Jie, Dong Fu-sheng, Dong Yu-ying, Hou Ya-li
Abstract excerpt
OBJECTIVE: To analyze the original mutated codon of p53 gene of salivary pleomorphic adenoma (SPA) and to evaluate the repair effects of wt-p53 on SPA cells. METHODS: Four cases of SPA were obtained from clinical fresh samples and SPA cells were separated and cultured, and then the cells were transduced by Ad-wt-p53. The cells and the corresponding tumor tissue DNA were extracted, PCR and single strand...
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