Article
Macronuclear transformation with specific DNA fragments controls the content of the new macronuclear genome in Paramecium tetraurelia.
Molecular and cellular biology - 1 Feb 1991
You Y, Aufderheide K, Morand J, Rodkey K, Forney J
Abstract excerpt
A previously isolated mutant cell line called d48 contains a complete copy of the A surface antigen gene in the micronuclear genome, but the gene is not incorporated into the macronucleus. Previous experiments have shown that a cytoplasmic factor made in the wild-type macronucleus can rescue the mutant. Recently, S. Koizumi and S. Kobayashi (Mol. Cell. Biol. 9:4398-4401, 1989) observed that injection of a plasmid...
Topics
- Animals
- Antigens, Protozoan
- Antigens, Surface
- Blotting, Southern
- Cell Nucleus
- Cloning, Molecular
- DNA
- Molecular Weight
- Mutation
- Nucleic Acid Hybridization
- Paramecium