Article
The IscA from Acidithiobacillus ferrooxidans is an iron-sulfur protein which assemble the [Fe4S4] cluster with intracellular iron and sulfur.
Archives of biochemistry and biophysics - 15 Jul 2007
Zeng Jia, Geng Meimei, Jiang Huidan, Liu Yuandong, Liu Jianshe, Qiu Guanzhou
Abstract excerpt
IscA was proposed to be involved in the iron-sulfur cluster assembly in Acidithiobacillus ferrooxidans encoded by the iscSUA operon, but the role of IscA in the iron-sulfur cluster assembly still remains controversial. In this study, the IscA from A. ferrooxidans ATCC 23270 was successfully expressed in Escherichia coli, and purified by affinity chromatography to homogeneity. To our surprise, the purified IscA...
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