An antisense RNA controls synthesis of an SOS-induced toxin evolved from an antitoxin.

Abstract excerpt

Only few small, regulatory RNAs encoded opposite another gene have been identified in bacteria. Here, we report the characterization of a locus where a small RNA (SymR) is encoded in cis to an SOS-induced gene whose product shows homology to the antitoxin MazE (SymE). Synthesis of the SymE protein is tightly repressed at multiple levels by the LexA repressor, the SymR RNA and the Lon protease. SymE co-purifies...

Topics

• Amino Acid Sequence
• Antitoxins
• Bacterial Toxins
• Base Sequence
• Blotting, Northern
• Electrophoresis, Polyacrylamide Gel
• Evolution, Molecular
• Immunoblotting
• Models, Molecular
• Molecular Sequence Data
• Mutation