Article
The lineaging of fluorescently-labeled Caenorhabditis elegans embryos with StarryNite and AceTree.
Nature protocols - 1 Jan 2006
Murray John Isaac, Bao Zhirong, Boyle Thomas J, Waterston Robert H
Abstract excerpt
Lineage analysis of Caenorhabditis elegans is a powerful tool for characterizing developmental phenotypes and embryonic gene-expression patterns. We present a detailed protocol for the lineaging of embryos by computational analysis of 4D images of embryos that ubiquitously express histone-GFP (green fluorescent protein) fusion proteins through the 350 cell stage followed by manual editing. We describe how to...
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