Human peritoneal mesothelial cells isolated from spent dialysate fluid maintain contaminating macrophages via production of macrophage colony stimulating factor.

doi:10.1111/j.1440-1797.2006.00760.x

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Article

Human peritoneal mesothelial cells isolated from spent dialysate fluid maintain contaminating macrophages via production of macrophage colony stimulating factor.

Nephrology (Carlton, Vic.) - 1 Apr 2007

Tee Melinda M, Tesch Gregory H, Nikolic-Paterson David J, Brown Fiona G

Abstract excerpt

BACKGROUND: Human peritoneal mesothelial cells (HPMC) are useful for the analysis of peritoneal reactions to various insults and to peritoneal dialysate. HPMC can be readily obtained from spent dialysis fluid, but leucocyte contamination is a major problem when using these cells for in vitro experiments. Therefore, we examined the persistence of leucocyte contamination in HPMC cultures obtained from spent...

Topics

Antigens, CD
Antigens, Differentiation, Myelomonocytic
Calbindin 2
Cell Proliferation
Cell Separation
Culture Media, Conditioned
Dialysis Solutions
Epithelial Cells
Flow Cytometry
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Keratin-18

Keratin-8

Leukocyte Common Antigens

Macrophage Colony-Stimulating Factor

Macrophages, Peritoneal

Paracrine Communication

Peritoneal Dialysis

Peritoneum

Source

Open in PubMed

Identifiers

PMID: 17371340
DOI: 10.1111/j.1440-1797.2006.00760.x

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