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Article

Differentiation of five tuna species by a multiplex primer-extension assay.

Journal of biotechnology - 1 May 2007

Bottero Maria Teresa, Dalmasso Alessandra, Cappelletti Marco, Secchi Camillo, Civera Tiziana

Abstract excerpt

A novel methodology based on analysis of mtDNA-cytb diagnostic sites was performed to discriminate four closely related species of Thunnus (Thunnus alalunga, Thunnus albacares, Thunnus obesus and Thunnus thynnus) and one species of Euthynnus (Katsuwonus pelamis) genus in raw and canned tuna. The primers used in the preliminary PCR designed in well conserved region upstream and downstream of the diagnosis sites...

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Identifiers

PMID: 17353060
DOI: 10.1016/j.jbiotec.2007.01.032

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