Article
Successful gene tagging in lettuce using the Tnt1 retrotransposon from tobacco.
Plant physiology - 1 May 2007
Mazier Marianne, Botton Emmanuel, Flamain Fabrice, Bouchet Jean-Paul, Courtial Béatrice, Chupeau Marie-Christine, Chupeau Yves, Maisonneuve Brigitte, Lucas Hélène
Abstract excerpt
The tobacco (Nicotiana tabacum) element Tnt1 is one of the few identified active retrotransposons in plants. These elements possess unique properties that make them ideal genetic tools for gene tagging. Here, we demonstrate the feasibility of gene tagging using the retrotransposon Tnt1 in lettuce (Lactuca sativa), which is the largest genome tested for retrotransposon mutagenesis so far. Of 10 different...
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