Article
Characterization of a loss-of-function mutant of gibberellin biosynthetic gene LsGA3ox1 in lettuce.
Bioscience, biotechnology, and biochemistry - 1 Jan 2011
Umetsu Asami, Sawada Yoshiaki, Mitsuhashi Wataru, Mazier Marianne, Toyomasu Tomonobu
Abstract excerpt
A previous study generated lettuce (Lactuca sativa) mutant lines tagged by retrotransposon Tnt1 from tobacco (Nicotiana tabacum) and identified a homozygous mutant, Tnt6a, that exhibited severe dwarf phenotype. Here we show that Tnt1 is inserted into the intron of gibberellin biosynthetic gene LsGA3ox1 in Tnt6a mutants. Expression analysis suggests that LsGA3ox1 is nearly knocked out in the Tnt6a mutants.
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