Article
Characterization of beta-xylosidase enzyme from a Pichia stipitis mutant.
Bioresource technology - 1 Jan 2008
Basaran Pervin, Ozcan Meltem
Abstract excerpt
beta-Xylosidase production was maximal for the mutant Pichia stipitis NP54376 grown on xylan as the sole carbon source. beta-Xylosidase was purified from culture supernatant by (NH(4))(2)SO(4) precipitation and a hydrophobic interaction chromatography on phenyl sepharose. Optima of pH and temperature were 5.0 and 50 degrees C, respectively. The enzyme was inhibited by 2-mercaptoethanol (100%) and Fe(3+) (80%),...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
