Article
A reverse transcription loop-mediated isothermal amplification assay optimized to detect multiple HIV subtypes.
PloS one - 1 Jan 2015
Ocwieja Karen E, Sherrill-Mix Scott, Liu Changchun, Song Jinzhao, Bau Haim, Bushman Frederic D
Abstract excerpt
Diagnostic methods for detecting and quantifying HIV RNA have been improving, but efficient methods for point-of-care analysis are still needed, particularly for applications in resource-limited settings. Detection based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is particularly useful for this, because when combined with fluorescence-based DNA detection, RT-LAMP can be implemented...
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