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Article

An improved cell-free system for picornavirus synthesis.

Journal of virological methods - 1 Jun 2007

Kobayashi Tominari, Mikami Satoshi, Yokoyama Shigeyuki, Imataka Hiroaki

Abstract excerpt

Cell-free synthesis of an infectious virus is an ideal tool for elucidating the mechanism of viral replication and for screening anti-viral drugs. In the present study, the synthesis of Encephalomyocarditis virus (EMCV) from its RNA in HeLa and 293-F cell extracts was enhanced by employing a dialysis system in combination with a ribozyme technology. Although translation and processing of the EMCV polyprotein were...

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Identifiers

PMID: 17320977
DOI: 10.1016/j.jviromet.2007.01.026

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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