Article
Sequence-specific 1H and 15N resonance assignments for human dihydrofolate reductase in solution.
Biochemistry - 14 Jan 1992
Stockman B J, Nirmala N R, Wagner G, Delcamp T J, DeYarman M T, Freisheim J H
Abstract excerpt
Dihydrofolate reductase is an intracellular target enzyme for folate antagonists, including the anticancer drug methotrexate. In order to design novel drugs with altered binding properties, a detailed description of protein-drug interactions in solution is desirable to understand the specificity of drug binding. As a first step in this process, heteronuclear three-dimensional NMR spectroscopy has been used to...
Topics
- Amino Acid Sequence
- Binding Sites
- Folic Acid
- Humans
- Hydrogen
- Magnetic Resonance Spectroscopy
- Methotrexate
- Molecular Sequence Data
- Mutation
- Nitrogen Isotopes
- Protein Conformation