Article
Improved fusion protein expression of EGFP via the mutation of both Kozak and the initial ATG codon.
Cellular & molecular biology letters - 1 Sept 2007
Dai Chao, Cao Zhijian, Wu Yingliang, Yi Hong, Jiang Dahe, Li Wenxin
Abstract excerpt
Since its discovery, green fluorescence protein (GFP) has been used as a reporter in a broad range of applications, including the determination of gene expresion in diverse organisms, and subcellular protein localization. pEGFP-N1 is a eukayotic expression vector encoding EGFP, the MCS of which l...
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