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Article

piggyBac-based insertional mutagenesis in Tribolium castaneum using donor/helper hybrids.

Insect molecular biology - 1 Jun 2007

Lorenzen M D, Kimzey T, Shippy T D, Brown S J, Denell R E, Beeman R W

Abstract excerpt

We describe an efficient method for generating new piggyBac insertions in the germline of F(1) hybrid Tribolium castaneum derived from crosses between transgenic helper and donor strains. Helper strains carried single Minos elements encoding piggyBac transposase. The donor strain carried a single piggyBac element inserted into an actin gene, expanding the eye-specific, 3xP3-EGFP (enhanced green fluorescent...

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Identifiers

PMID: 17316329
DOI: 10.1111/j.1365-2583.2007.00727.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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