Article
Structure function relationships in the ribosomal stalk proteins of archaebacteria.
The Journal of biological chemistry - 15 Jan 1992
Köpke A K, Leggatt P A, Matheson A T
Abstract excerpt
The ribosomal L12 protein gene of Sulfolobus solfataricus (SsoL12) has been subcloned and overexpressed in Escherichia coli. Five protein L12 mutants were designed: two NH2-terminal and two COOH-terminal truncated mutants and one mutant lacking the highly charged part of the COOH-terminal region. The mutant protein genes were overexpressed in E. coli and the products purified. The amino acid composition was...
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