Article
Structure function relationships in the ribosomal stalk proteins of archaebacteria.
The Journal of biological chemistry - 15 Jan 1992
Köpke A K, Leggatt P A, Matheson A T
Abstract excerpt
The ribosomal L12 protein gene of Sulfolobus solfataricus (SsoL12) has been subcloned and overexpressed in Escherichia coli. Five protein L12 mutants were designed: two NH2-terminal and two COOH-terminal truncated mutants and one mutant lacking the highly charged part of the COOH-terminal region....
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Electrophoresis, Agar Gel
- Electrophoresis, Gel, Two-Dimensional
- Molecular Sequence Data
- Mutation
- Poly U
- Protein Biosynthesis
- Ribosomal Proteins
- Ribosomes
- Structure-Activity Relationship
- Sulfolobus
- Ultracentrifugation