Article
Differential effects of replacing Escherichia coli ribosomal protein L27 with its homologue from Aquifex aeolicus.
Journal of bacteriology - 1 Nov 2001
Maguire B A, Manuilov A V, Zimmermann R A
Abstract excerpt
The rpmA gene, which encodes 50S ribosomal subunit protein L27, was cloned from the extreme thermophile Aquifex aeolicus, and the protein was overexpressed and purified. Comparison of the A. aeolicus protein with its homologue from Escherichia coli by circular dichroism analysis and proton nuclear magnetic resonance spectroscopy showed that it readily adopts some structure in solution that is very stable, whereas...
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