Article
Cloning, mutagenesis, and physiological effect of a hydroxypyruvate reductase gene from Methylobacterium extorquens AM1.
Journal of bacteriology - 1 Jan 1992
Chistoserdova L V, Lidstrom M E
Abstract excerpt
The gene encoding the serine cycle hydroxypyruvate reductase of Methylobacterium extorquens AM1 was isolated by using a synthetic oligonucleotide with a sequence based on a known N-terminal amino acid sequence. The cloned gene was inactivated by insertion of a kanamycin resistance gene, and recombination of this insertion derivative with the wild-type gene produced a serine cycle hydroxypyruvate reductase null...
Topics
- Alcohol Oxidoreductases
- Base Sequence
- Cloning, Molecular
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Glycine Hydroxymethyltransferase
- Glyoxylates
- Gram-Negative Aerobic Bacteria
- Hydroxypyruvate Reductase
- Isoelectric Point