Article
Construction of insertion and deletion mxa mutants of Methylobacterium extorquens AM1 by electroporation.
FEMS microbiology letters - 1 Sept 1998
Toyama H, Anthony C, Lidstrom M E
Abstract excerpt
Methylobacterium extorquens AM1 is a pink-pigmented facultative methylotroph which is widely used for analyzing pathways of C1 metabolism with biochemical and molecular biological techniques. To facilitate this approach, we have applied a new method to construct insertion or disruption mutants wi...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Chromosome Mapping
- DNA, Bacterial
- Electroporation
- Genes, Bacterial
- Genetic Complementation Test
- Gram-Negative Aerobic Rods and Cocci
- Methanol
- Molecular Sequence Data
- Multigene Family
- Mutagenesis, Insertional
- Mutation
- Plasmids
- Recombination, Genetic
- Sequence Deletion