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Article

Construction of insertion and deletion mxa mutants of Methylobacterium extorquens AM1 by electroporation.

FEMS microbiology letters - 1 Sept 1998

Toyama H, Anthony C, Lidstrom M E

Abstract excerpt

Methylobacterium extorquens AM1 is a pink-pigmented facultative methylotroph which is widely used for analyzing pathways of C1 metabolism with biochemical and molecular biological techniques. To facilitate this approach, we have applied a new method to construct insertion or disruption mutants with drug resistance genes by electroporation. By using this method, mutants were obtained in four genes present in the...

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PMID: 9741078
DOI: 10.1111/j.1574-6968.1998.tb13175.x

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