Poly-beta-hydroxybutyrate biosynthesis in the facultative methylotroph methylobacterium extorquens AM1: identification and mutation of gap11, gap20, and phaR.

Abstract excerpt

Methylobacterium extorquens AM1, a serine cycle facultative methylotroph, accumulates poly-beta-hydroxybutyrate (PHB) as a carbon and energy reserve material during growth on both multicarbon- and single-carbon substrates. Recently, the identification and mutation of the genes involved in the biosynthesis and degradation of PHB have been described for this bacterium, demonstrating that two of the genes of the PHB...

Topics

• Acetyl Coenzyme A
• Bacterial Proteins
• Culture Media
• DNA, Bacterial
• DNA-Binding Proteins
• Gene Expression Regulation, Bacterial
• Hydroxybutyrates
• Methanol
• Methylobacterium extorquens
• Molecular Sequence Data
• Mutagenesis, Site-Directed
• Mutation
• Polyesters