Article
Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene.
Nucleic acids research - 1 Jan 2007
Chun Jong-Yoon, Kim Kyoung-Joong, Hwang In-Taek, Kim Yun-Jee, Lee Dae-Hoon, Lee In-Kyoung, Kim Jong-Kee
Abstract excerpt
Successful PCR starts with proper priming between an oligonucleotide primer and the template DNA. However, the inevitable risk of mismatched priming cannot be avoided in the currently used primer system, even though considerable time and effort are devoted to primer design and optimization of reaction conditions. Here, we report a novel dual priming oligonucleotide (DPO) which contains two separate priming...
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