Article
Isolation and molecular characterization of a Spotted leaf 18 mutant by modified activation-tagging in rice.
Plant molecular biology - 1 Apr 2007
Mori Masaki, Tomita Chikako, Sugimoto Kazuhiko, Hasegawa Morifumi, Hayashi Nagao, Dubouzet Joseph G, Ochiai Hirokazu, Sekimoto Hitoshi, Hirochika Hirohiko, Kikuchi Shoshi
Abstract excerpt
A lesion mimic mutant that we designated Spotted leaf 18 (Spl18) was isolated from 13,000 activation-tagging lines of rice produced by our modified activation-tagging vector and further characterized. Spl18 was dominant and its phenotype was linked to the T-DNA insertion. An ORF was located about 500 bp downstream of the inserted T-DNA, and the deduced protein, designated OsAT1, showed sequence similarity to an...
Topics
- Acyltransferases
- Amino Acid Sequence
- Blotting, Northern
- Gene Expression Regulation, Plant
- Magnaporthe
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Oryza
- Phylogeny