Article
Modulations of the epithelial phenotype and functional activity of cultured bovine tracheal gland cells: dependence on the culture medium and passage number.
Biology of the cell - 1 Jan 1991
Benali R, Dupuit F, Chevillard M, Jacquot J, Haye B, Puchelle E
Abstract excerpt
Bovine tracheal gland (BTG) cells in culture show an epithelial-fibroblastoid transition after several passages. To investigate these BTG cell phenotype changes, we studied the effects of both the culture medium and passage number on the expression of epithelial cytoskeletal proteins and glandular serous cell markers. We also analyzed the intracellular cAMP level in the basal state and after adrenergic...
Topics
- 1-Methyl-3-isobutylxanthine
- Animals
- Cattle
- Cell Differentiation
- Cells, Cultured
- Culture Media
- Culture Techniques
- Cyclic AMP
- Cytoskeletal Proteins
- Desmoplakins
- Epithelial Cells
