Article
Comparison of DNA extraction methods for analysis of turkey cecal microbiota.
Journal of applied microbiology - 1 Feb 2007
Scupham A J, Jones J A, Wesley I V
Abstract excerpt
AIM: As a prelude to long-term studies to characterize the microbiota of the turkey ceca, 14 DNA isolation protocols were evaluated for their ability to reproducibly characterize microbial diversity. METHODS AND RESULTS: Eight commercially available DNA extraction kits were assessed. DNA quantity and quality were assessed and competitive PCR was used to quantify the 16S bacterial rRNA genes. The Invitrogen...
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