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Article

Assay for three-way interaction of protein phosphatase-1 (Glc7) with regulatory subunits plus phosphatase inhibitor-2.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007

Eto Masumi, Brautigan David L

Abstract excerpt

A method is described using yeast conjugation to assay the interactions of a protein phosphatase-1 (PP1) inhibitor protein with holoenzymes formed in situ by expression of regulatory subunit fusion proteins that recruit endogenous Glc7, the yeast ortholog of PP1. Mutations in the canonical recognition motif VxF used to bind PP1 (Glc7) allow for analysis of direct from indirect (three-way) interactions.

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Identifiers

PMID: 17200563
DOI: 10.1385/1-59745-267-X:197

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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