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Article

Pseudouridine modification of U5 RNA in ribonucleoprotein particles assembled in vitro.

Molecular and cellular biology - 1 Dec 1991

Patton J R

Abstract excerpt

The formation of pseudouridine (psi) in U5 RNA during ribonucleoprotein (RNP) assembly was investigated by using HeLa cell extracts. In vitro transcribed, unmodified U5 RNA assembled into an RNP particle with the same buoyant density and sedimentation velocity as did U5 small nuclear RNP from extracts. The greatest amount of psi modification was detected when a combination of S100 and nuclear extracts was used...

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Identifiers

PMID: 1719377
DOI: 10.1128/mcb.11.12.5998-6006.1991
PMCID: PMC361764

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