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Culture of human pluripotent stem cells using completely defined conditions on a recombinant E-cadherin substratum

2 Jun 2010

Abstract excerpt

BACKGROUND: To maintain pluripotency of human embryonic stem (huES) cells in feeder-free culture it has been necessary to provide a Matrigel substratum, which is a complex of poorly defined extracellular matrices and growth factors derived from mouse Engelbreth-Holm-Swarm sarcoma cells. Culture of stem cells under ill-defined conditions can inhibit the effectiveness of maintaining cells in a pluripotent state and...

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PMID: 20525219
DOI: 10.1186/1471-213x-10-60

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