Article
Analysis of genetic markers of N. gonorrhoeae resistance to beta-lactam antibiotics.
Bulletin of experimental biology and medicine - 1 May 2006
Malakhova M V, Vereshchagin V A, Il'ina E N, Govorun V M, Zubkov M M, Priputnevich T V, Kisina V I, Kubanova A A
Abstract excerpt
A complex method for detection of genetic markers of N. gonorrhoeae resistance to penicillin was developed. Mutations in penA and ponA genes were detected by minisequencing reaction with subsequent detection of reaction products by MALDI-TOF mass spectrometry. This approach was tested on 31 clinical strains of N. gonorrhoeae with minimum inhibitory concentration of penicillin from 0.03 to 8 microg/ml and higher....
Topics
- Bacterial Proteins
- DNA Primers
- Genetic Markers
- Microbial Sensitivity Tests
- Mutation
- Neisseria gonorrhoeae
- Penicillin-Binding Proteins
- Penicillins
- Sequence Analysis, DNA
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization