Article
Consistence of miniexon polymerase chain reaction-restriction fragment length polymorphism and single-copy gene sequence analyses in discriminating Leishmania genotypes.
Diagnostic microbiology and infectious disease - 1 Mar 2007
Serin Mehmet S, Waki Kayoko, Chang Kwang-Poo, Aslan Gonul, Direkel Sahin, Otag Feza, Kayar Begum, Koksal Fatih, Emekdas Gurol
Abstract excerpt
Recently, a new polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP)-based assay had been developed using the miniexon sequences for genotyping Leishmania isolates. We had used this method for rapid diagnosis and genotyping of visceral and cutaneous leishmaniasis with the combination of microcapillary cultivation. In this study, we have evaluated this approach by examining genomic DNAs...
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