Article
Assembly of the surfactant protein SP-A. Deletions in the globular domain interfere with the correct folding of the molecule.
European journal of biochemistry - 1 Jul 1991
Spissinger T, Schäfer K P, Voss T
Abstract excerpt
The C-terminal non-collagenous domain of the surfactant glycoprotein SP-A was shown to be essential for its correct folding and assembly, as judged by the secretion of various deletion mutants transiently expressed in COS cells. A deletion mutant coding for this domain was successfully secreted w...
Topics
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Cell Line
- Chromatography, High Pressure Liquid
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Fluorescent Antibody Technique
- Glycoproteins
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation
- Proteolipids
- Pulmonary Surfactant-Associated Protein A
- Pulmonary Surfactant-Associated Proteins
- Pulmonary Surfactants
- RNA