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Article

Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase.

Journal of bacteriology - 1 Jun 1991

Sun D X, Setlow P

Abstract excerpt

L-Aspartase was purified from Bacillus subtilis, its N-terminal amino acid sequence was determined to construct a probe for the aspartase gene, and the gene (termed ansB) was cloned and sequenced. A second gene (termed ansA) was found upstream of the ansB gene and coded for L-asparaginase. These two genes were in an operon designated the ans operon, which is 80% cotransformed with the previously mapped aspH1...

Topics

Amino Acid Sequence
Asparaginase
Aspartate Ammonia-Lyase
Bacillus subtilis
Base Sequence
Blotting, Northern
Chromatography, Liquid
Chromosomes, Bacterial
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Gene Expression Regulation, Bacterial

Gene Expression Regulation, Enzymologic

Genes, Bacterial

Molecular Sequence Data

Mutation

Operon

RNA, Bacterial

RNA, Messenger

Source

Open in PubMed Open full text

Identifiers

PMID: 1711029
DOI: 10.1128/jb.173.12.3831-3845.1991
PMCID: PMC208015

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1711029-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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