Article
Mapping of the T and B cell epitopes of the Mycobacterium bovis protein, MPB70.
Immunology and cell biology - 1 Dec 1990
Billman-Jacobe H, Radford A J, Rothel J S, Wood P R
Abstract excerpt
A clone coding for the entire gene for the Mycobacterium bovis protein antigen MPB70 was used to produce a series of overlapping subclones by making a series of deletions from the 3' end of the gene. The subclones expressed incomplete MPB70 proteins as fusions with glutathione-S-transferase. The insert DNA was sequenced to determine the extent of the deletion and the proteins expressed by the clones were examined...
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