Article
Mapping of the T and B cell epitopes of the Mycobacterium bovis protein, MPB70.
Immunology and cell biology - 1 Dec 1990
Billman-Jacobe H, Radford A J, Rothel J S, Wood P R
Abstract excerpt
A clone coding for the entire gene for the Mycobacterium bovis protein antigen MPB70 was used to produce a series of overlapping subclones by making a series of deletions from the 3' end of the gene. The subclones expressed incomplete MPB70 proteins as fusions with glutathione-S-transferase. The insert DNA was sequenced to determine the extent of the deletion and the proteins expressed by the clones were examined...
Topics
- Amino Acid Sequence
- Animals
- Antigens, Bacterial
- B-Lymphocytes
- Bacterial Proteins
- Base Sequence
- Cattle
- Chromosome Deletion
- Cloning, Molecular
- Enzyme-Linked Immunosorbent Assay
- Epitopes