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Article

Development and Application of a Real‐Time Quantitative PCR for Prenatal Detection of Fetal α0‐Thalassemia from Maternal Plasma

1 Sept 2006

Abstract excerpt

In order to provide a noninvasive prenatal diagnosis of alpha(0)-Thalassemia (Southeast Asian [SEA] deletion), we have developed a real-time quantitative semi-nested polymerase chain reaction (PCR) method for identifying the fetal alpha(0)-Thalassemia in maternal plasma. Analysis was performed using DNA extracted from 200 muL plasma from 13 pregnant women during 8-20 weeks of gestation who carried fetuses with...

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Identifiers

PMID: 17108198
DOI: 10.1196/annals.1368.013

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