Article
Semi-nested Taqman real-time quantitative PCR for noninvasive prenatal diagnosis of Bart's hydrops fetalis.
Journal of the Medical Association of Thailand = Chotmaihet thangphaet - 1 Jan 2012
Pornprasert Sakorn, Sukunthamala Kanyakan, Kunyanone Naowarat, Sittiprasert Sririchai, Thungkham Khanungnit, Junorse Sumeth, Pongsawatkul Khachonsilp, Pattanaporn Wisut, Jitwong Chantip
Abstract excerpt
BACKGROUND: Non-invasive prenatal diagnosis based on detection of fetal cell-free DNA is limited when mother and father are both carriers for the same autosomal recessive mutation. OBJECTIVE: Develop the semi-nested Taqman real-time PCR for quantification of alpha-thalassemia-1 SEA type deletion allele in plasma of alpha-thalassemia-1 SEA carriage pregnancies. MATERIAL AND METHOD: Plasma DNA was extracted from...
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