Article
Heme concentration dependence and metalloporphyrin inhibition of the system I and II cytochrome c assembly pathways.
Journal of bacteriology - 1 Jan 2007
Richard-Fogal Cynthia L, Frawley Elaine R, Feissner Robert E, Kranz Robert G
Abstract excerpt
Studies have indicated that specific heme delivery to apocytochrome c is a critical feature of the cytochrome c biogenesis pathways called system I and II. To determine directly the heme requirements of each system, including whether other metal porphyrins can be incorporated into cytochromes c, we engineered Escherichia coli so that the natural system I (ccmABCDEFGH) was deleted and exogenous porphyrins were the...
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