Article
Repression and derepression of conjugation of plasmid R1 by wild-type and mutated finP antisense RNA.
Molecular microbiology - 1 Jan 1991
Koraimann G, Koraimann C, Koronakis V, Schlager S, Högenauer G
Abstract excerpt
The finP gene of plasmid R1 is located between the genes traM and traJ, partially overlapping the first few nucleotides of the latter. It codes for a repressor of the conjugation system. The product of this gene is a small RNA of 72 nucleotides and, because it is transcribed from the opposite DNA strand, it is complementary to the 5' non-translated sequences, the ribosome-binding site, and the first two codons of...
Topics
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- Conjugation, Genetic
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genes, Synthetic
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation