Article
A series of ENU-induced single-base substitutions in a long-range cis-element altering Sonic hedgehog expression in the developing mouse limb bud.
Genomics - 1 Feb 2007
Masuya Hiroshi, Sezutsu Hideki, Sakuraba Yoshiyuki, Sagai Tomoko, Hosoya Masaki, Kaneda Hideki, Miura Ikuo, Kobayashi Kimio, Sumiyama Kenta, Shimizu Aya, Nagano Junko, Yokoyama Haruka, Kaneko Satoko, Sakurai Noriko, Okagaki Yuka, Noda Tetsuo, Wakana Shigeharu, Gondo Yoichi, Shiroishi Toshihiko
Abstract excerpt
Mammal-fish-conserved-sequence 1 (MFCS1) is a highly conserved sequence that acts as a limb-specific cis-acting regulator of Sonic hedgehog (Shh) expression, residing 1 Mb away from the Shh coding sequence in mouse. Using gene-driven screening of an ENU-mutagenized mouse archive, we obtained mice with three new point mutations in MFCS1: M101116, M101117, and M101192. Phenotype analysis revealed that M101116 mice...
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