Article
A nucleic acid sequence-based amplification assay for real-time detection of norovirus genogroup II.
Journal of applied microbiology - 1 Oct 2006
Patterson S S, Smith M W, Casper E T, Huffman D, Stark L, Fries D, Paul J H
Abstract excerpt
AIMS: To use molecular beacon based nucleic acid sequence-based amplification (NASBA) to develop a rapid, sensitive, specific detection method for norovirus (NV) genogroupII (GII). METHODS AND RESULTS: A method to detect NV GII from environmental samples using real-time NASBA was developed. This method was routinely sensitive to 100 copies of target RNA and intermittent amplification occurred with as few as 10...
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