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NUCLEIC ACID SEQUENCE-BASED AMPLIFICATION (NASBA) IN MOLECULAR BACTERIOLOGY: A PROCEDURAL GUIDE

2007-09-01

Abstract excerpt

As a means of assessing gene expression and RNA structure/function, nucleic acid sequence-based amplification (NASBA) involves an isothermic series of reactions using avian myeloblastosis virus reverse transcriptase (RT), RNase H, T7 RNA polymerase, transcript-specific primers and associated cofactors to amplify large amounts of target RNA. NASBA offers greater speed, sensitivity and versatility compared to other...

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Literature Corpus work
69b707d4-a687-5a1b-a12f-72616994d099
DOI
10.1111/j.1745-4581.2007.00099.x
Open publication

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