Article
Rapid preimplantation detection of mutant (shiverer) and normal alleles of the mouse myelin basic protein gene allowing selective implantation and birth of live young.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1990
Gomez C M, Muggleton-Harris A L, Whittingham D G, Hood L E, Readhead C
Abstract excerpt
As a model for the detection of human genetic disease in preimplantation embryos, we describe a method in which trophectoderm biopsy samples from viable mouse blastocysts are simultaneously analyzed for the presence of a normal or mutant allele of the myelin basic protein gene by the polymerase c...
Topics
- Alleles
- Animals
- Base Sequence
- Biopsy
- Blastocyst
- Cells, Cultured
- Ectoderm
- Embryo Implantation
- Exons
- Female
- Genes
- Mice
- Mice, Neurologic Mutants
- Molecular Sequence Data
- Mutation