Article
BRAF, K-ras and BAT26 mutations in colorectal polyps and stool.
World journal of gastroenterology - 28 Aug 2006
Jin Ying-Min, Li Bao-Jie, Qu Bo, Du Ya-Ju
Abstract excerpt
AIM: To assess the feasibility of using BRAF, K-ras and BAT26 genes as stool-based molecular markers for detection of colorectal adenomas and hyperplastic polyps (HPs). METHODS: We applied PCR-SSCP and direct sequencing to detect BRAF mutations of polyps and paired stool samples. Primer-mediated restriction fragment length polymorphism (RFLP) analysis and mutant-enriched PCR were used in detection of K-ras...
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