Article
Cloning of a chicken liver cDNA encoding 5-aminoimidazole ribonucleotide carboxylase and 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase by functional complementation of Escherichia coli pur mutants.
Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990
Chen Z D, Dixon J E, Zalkin H
Abstract excerpt
We have used functional complementation of Escherichia coli pur mutants to clone avian cDNA encoding 5-aminoimidazole ribonucleotide (AIR) carboxylase-5-aminoimidazole-4-N-succinocarboxamide ribonucleotide (SAICAR) synthetase, the bifunctional enzyme catalyzing steps 6 and 7 in the pathway for de...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Carboxy-Lyases
- Chickens
- Cloning, Molecular
- Escherichia coli
- Genetic Complementation Test
- Liver
- Molecular Sequence Data
- Mutation
- Peptide Synthases
- Plasmids
- RNA
- Restriction Mapping