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Real-time PCR detection and speciation of Cryptosporidium infection using Scorpion probes

16 Aug 2006

Abstract excerpt

At least eight species of Cryptosporidium can cause human infection and disease. A real-time PCR (qPCR) assay based on the 18S rRNA gene and utilizing a Scorpion probe was developed to detect all human-pathogenic Cryptosporidium without the usual need for nested amplification. Sensitivity of detection in stool samples was highest using a glass bead-based DNA extraction method (under 10(3) oocysts per stool...

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PMID: 16914651
DOI: 10.1099/jmm.0.46678-0

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