Article
Identification of Cryptosporidium species and genotypes in Scottish raw and drinking waters during a one-year monitoring period.
Applied and environmental microbiology - 1 Sept 2010
Nichols R A B, Connelly L, Sullivan C B, Smith H V
Abstract excerpt
We analyzed 1,042 Cryptosporidium oocyst-positive slides (456 from raw waters and 586 from drinking waters) of which 55.7% contained 1 or 2 oocysts, to determine species/genotypes present in Scottish waters. Two nested PCR-restriction fragment length polymorphism (RFLP) assays targeting different loci (1 and 2) of the hypervariable region of the 18S rRNA gene were used for species identification, and 62.4% of...
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