Article
Multiplex real-time PCR assay using Scorpion probes and DNA capture for genotype-specific detection of Giardia lamblia on fecal samples.
Journal of clinical microbiology - 1 Mar 2005
Ng Cherie T, Gilchrist Carol A, Lane Ariel, Roy Shantanu, Haque Rashidul, Houpt Eric R
Abstract excerpt
Two major genotypic assemblages of Giardia lamblia infect humans; the epidemiologic significance of this phenomenon is poorly understood. We developed a single-vessel multiplex real-time PCR (qPCR) assay that genotypes Giardia infections into assemblages A and/or B directly from fecal samples. The assay utilized Scorpion probes that combined genotype-specific primers and probes for the 18S rRNA gene into the same...
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