Article
The RNA antiterminator causes transcription pausing in the leader region of the tryptophan operon.
The Journal of biological chemistry - 15 Apr 1990
Roesser J R, Yanofsky C
Abstract excerpt
In vitro transcription studies with a trp leader DNA template derived from a double deletion mutant of Serratia marcescens revealed that the transcription complex pauses synthesis of part of the RNA antiterminator, structure 2:3. Pausing was enhanced by NusA protein and was dependent on the concentration of UTP in the transcription reaction mixture. A weak antiterminator pause also was detected during...
Topics
- Base Sequence
- Chromosome Deletion
- Genes, Regulator
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Operon
- Plasmids
- Protein Sorting Signals
- RNA, Bacterial