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Article

The effect of the hexahistidine-tag in the oligomerization of HSC70 constructs.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 5 Dec 2006

Amor-Mahjoub Mouna, Suppini Jean-Philippe, Gomez-Vrielyunck Nathalie, Ladjimi Moncef

Abstract excerpt

The hexahistidine is a fusion tag used for the isolation of proteins via an immobilized metal-ion affinity chromatography (IMAC). In the present study, we have purified and analyzed two constructs of the heat shock protein HSC70 in the presence or the absence of the His-tag (C30WT-His(+)/C30WT and C30DeltaL-His(+)/C30DeltaL). The oligomerization properties of the constructs were analyzed by size exclusion...

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Identifiers

PMID: 16904956
DOI: 10.1016/j.jchromb.2006.07.031

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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