Article
Some properties of human small heat shock protein Hsp20 (HspB6).
European journal of biochemistry - 1 Jan 2004
Bukach Olesya V, Seit-Nebi Alim S, Marston Steven B, Gusev Nikolai B
Abstract excerpt
Human heat shock protein of apparent molecular mass 20 kDa (Hsp20) and its mutant, S16D, mimicking phosphorylation by cyclic nucleotide-dependent protein kinases, were cloned and expressed in Escherichia coli. The proteins were obtained in a homogeneous state without utilization of urea or detergents. On size exclusion chromatography at neutral pH, Hsp20 and its S16D mutant were eluted as symmetrical peaks with...
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