Article
Novel monoclonal antibodies identify antigenic determinants unique to cellular senescence.
Journal of cellular physiology - 1 Feb 1990
Porter M B, Pereira-Smith O M, Smith J R
Abstract excerpt
Normal human diploid fibroblasts exhibit a limited lifespan in vitro and are used as a model to study in vivo aging. Monoclonal antibodies were generated against partially purified surface membranes from human diploid fibroblasts at the end of their lifespan (senescent). Three hybridomas were isolated that secreted antibodies reacting to cellular determinants expressed specifically on senescent human fibroblasts...
Topics
- Aging
- Antibodies, Monoclonal
- Biomarkers
- Blotting, Western
- Cell Line
- Cell Survival
- Diploidy
- Embryo, Mammalian
- Epitopes
- Fibroblasts
- Fibronectins
- Humans